Fig. 3

Validation of selected miRNA expression patterns. 25 miRNAs were found to be triple differentially expressed and are displayed as heat map (a). The heat map presents dCT data (dCT = CT (target gene) – CT (housekeeping gene)) with red indicating high (min. dCT value: - 2.4) and blue indicating low (max. dCT value: 11.1) expression (3-color scale with white representing the midpoint). Three of these genes characterized as triple differentially expressed were selected and analyzed by single TaqMan PCR assay PCR (b). Single PCR assays referred to in B were run on the same samples that had been analyzed on the TLDA card (n (LM) = 3, n (PER) = 3, n (M0) = 3) thus to show that the data deriving from the TLDA cards (referred to as “TLDA” in (b)) can be reproduced by running single assays (referred to as “single TaqMan assay” in (b)) on the cycler. Finally, expression of hsa-miR-483-5p, hsa-miR-31-5p and hsa-miR-215-5p was analyzed in the whole study cohort (n (LM) = 10, n (PER) = 10, c). Error bars represent standard error of mean. Significant changes in c (p < 0.05) are indicated with stars (*)