Skip to main content

Table 1 Metabolites affected in the cell supernatant

From: Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM

Pathway Metabolite 1D GC-MS p-value 2D GC-MS p-value AA Analysis p-value
Alanine And Aspartate Metabolism Aspargine 0.000    0.002
Alanine 0.000    0.007
Aspartic Acid    0.029 0.135
Amino Sugar Metabolism N-Acetyl Glucosamine 0.000     
N-Acetyl Mannosamine 0.000     
Beta-Alanine Metabolism Pantothenic Acid 0.292 0.009   
Citric Acid Cycle Succinic Acid 0.010     
Creatine Metabolism Creatinine 0.003     
Cystein      0.121
Cysteine And Methionine Metabolism Cystein (2 Derivative)      0.005
Cystine 0.005    0.003
Methionine 0.003    0.044
Fatty Acid Biosynthesis 3-Hydroxybutyric Acid    0.004   
Butanoic Acid    0.000   
Hexadecanoic Acid 0.200     
Fructose-1- Phosphate 0.050     
Fructose And Mannose Degradation Mannose 0.020     
Fructose 0.011     
Galactose Metabolism Galactose 0.225     
Glutamate Metabolism Glutamic Acid 0.000    0.247
GABA      0.001
Glutamine 0.000 0.009   
Glutathione Metabolism Pyroglutamic Acid 0.000     
Glycerolipid Metabolism Ethanolamine 0.010     
Glycine, Serine And Threonine Metabolism Glycine 0.068 0.012 0.152
Sarcosine 0.000     
Serine    0.007 0.020
Threonine 0.017    0.155
Glycolysis. Gluconeogenesis. Glucose 0.143     
Pyruvate Metabolism Glyceric Acid 0.000     
Lactic Acid 0.002     
Malic Acid 0.039     
Histidine Metabolism Histidine    0.033 0.027
Homocysteine Degradation 3-Methyl-2-Ketobutyric Acid    0.000   
Inositol Metabolism Inositol 0.002     
Lysine Metabolism Lysine 0.044 0.008 0.191
Lysine (2 Derivative)      0.027
Nicotinate And Nicotinamide Metabolism Nicotinamide 0.021 0.001   
Arabinose 0.003     
Nucleotide Sugar. Pentose Metabolism Arabitol 0.000     
Nucleotide Sugars Metabolism Xylose 0.004     
Pentose Phosphate Pathway Gluconic Acid 1.4-Lactone 0.034     
Ribofuranose    0.013   
Ribose 0.000 0.028   
Sedoheptulose-7-Phosphate 0.276     
Phenylalanine Metabolism Phenylalanine 0.000 0.022   
Purine Metabolism Allantoin 0.051     
Pyrimidine Metabolism Uracil 0.024     
Riboflavin Metabolism Ribitol 0.000     
Sugar. Sugar Substitute. Starch Erythritol 0.002     
Tryptophan Metabolism Tryptophan      0.035
Tyrosine Metabolism Tyrosine 0.034 0.010 0.052
Urea Cycle. Arginine and Proline Metabolism Ammonia      0.038
Arginine 0.015 0.010 0.070
(Arginine biosynthetic pathway) Argininosuccinate 0.021     
Asymetrical-N.N- Dimethylarginine 0.018     
Citrulline      0.098
Citrulline (Arginine) 0.016     
Citrulline (Ornthine) 0.019     
Hydroxyproline      
Ornithine (2 Derivative)      0.000
Ornithine 0.006    0.156
Proline 0.000    0.000
Urea 0.058 0.001   
2-Keto-3-Methylvaleric Acid    0.002   
Valine. Leucine And Isoleucine Degradation 2-Oxoisocaproic Acid 0.002 0.000   
Isoleucine 0.008 0.033 0.373
Leucine 0.056    0.253
Valine 0.010 0.002 0.132
Other 2-Aminobutyric Acid 0.000     
2-Deoxy-Galactose 0.002     
Allothreonine 0.010 0.000   
Cellotriose 0.224     
Ellagic Acid 0.293     
Gluconic Acid 0.095     
Isoerythritol 0.000     
Mannitol 0.031     
Pentanoic Acid    0.000   
Propanoic Acid    0.001   
Silanamine    0.021   
Sorbose 0.002     
Xylitol 0.000     
  1. ↑ Elevated in ASS+ve cell lines, ↓ lowered in ASS+ve cell lines. p-values was calculated using a Mann-Whitney U-test