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Fig. 6 | BMC Cancer

Fig. 6

From: ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer

Fig. 6

Through regulating miR-590-3p/GOLPH3 signaling pathway, can ATF-3 represses proliferation of breast cancer cells. a Ectopics expression of ATF-3 inhibited the expression of miR-590-3p. Data shown are means ± SD (n = 5), **P < 0.01 (b) MTS proliferation assay showed overexpression of ATF-3 could rescue miR-590-3p repressing cell proliferation during 24, 48, 72 h in MDA-MB-231. Data shown are means ± SD (n = 3), **P < 0.01. c BrdU incorporation assay. Data shown are means ± SD (n = 3), *P < 0.05. d Flow cytometry assay showed overexpression ATF-3 rescued the G1 phase arrest caused by miR-590-3p. Data shown are means ± SD (n = 5).e Expression level of mRNA of cell cycle-related genes. Data shown are means ± SD (n = 3). f MTS proliferation assay showed that knockdown of ATF-3 repressed cell proliferation, which could be rescued by overexpressing GOLPH3 during 24, 48, 72 h in MDA-MB-231. Data shown are means ± SD (n = 3), *P < 0.05, **P < 0.01. g BrdU incorporation assay. Data shown are means ± SD (n = 3), **P < 0.01. h Flow cytometry assay showed overexpression GOLPH3 rescued the influence of cell cycle caused by knockdown of ATF-3. Data shown are means ± SD (n = 5). i Expression level of mRNA of cell cycle-related genes. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01 versus the corresponding control

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