Fig. 5
From: ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer
Knockdown of ATF-3 inhibits the proliferation of breast cancer cells. a MTS proliferation assay showed downregulation of ATF-3 could inhibit proliferation during 24, 48, 72 h in MDA-MB-231. Data shown are means ± SD (n = 5), *P < 0.05. b BrdU incorporation assay. Data shown are means ± SD (n = 3), **P < 0.01. c Flow cytometry assay showed the increase of cell proportion in G1 phase of the ATF-3 knockdown group. Data shown are means ± SD (n = 5). d Detection of expression cell cycle-related genes by qPCR. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus the corresponding control