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Fig. 3 | BMC Cancer

Fig. 3

From: ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer

Fig. 3

miR-590-3p represses breast cancer cells proliferation and directly targets GOLPH3. a The GOLPH3 3′UTR reporter in target validation of miR-590-3p. b Luciferase analysis showed the inhibitory effect of miR-590-3p on expression of the luciferase reporter gene by binding GOLPH3 3’UTR. **P < 0.01. c q-PCR and western blot showed the down-regulated GOLPH3 protein in MDA-MB-231 by overexpressing miR-590-3p. Data shown are means ± SD (n = 3). **P < 0.01. d MTS assay showed miR-590-3p repressed cell proliferation during 24, 48, 72 h. Data shown are means ± SD (n = 3).**P < 0.01. e BrdU incorporation assay. Data shown are means ± SD (n = 5). **P < 0.01. f Flow cytometry assay showed the cell cycle arrest at G1 phase by overexpressing miR-590-3p. Data shown are means ± SD (n = 5). g Expression level of mRNA of cell cycle-related genes. Data shown are means ± SD (n = 3). *P < 0.05, ***P < 0.005 versus the corresponding control

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