Fig. 2

GOLPH3 regulates cell proliferation and cell cycle. a MTS assay showed promotion of cells proliferation by GOLPH3 overexpression during 24, 48, 72 h in MDA-MB-231. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01. b BrdU incorporation assay showed the promotion of proliferation by overexpressing GOLPH3 in MDA-MB-231. Data shown are means ± SD (n = 3). **P < 0.01. c Flow cytometry assay showed the regulation of cell cycle by overexpressing GOLPH3. Data shown are means ± SD (n = 5). d Expression level of mRNA of cell cycle-related genes. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01 versus the corresponding control. e Knockdown of GOLPH3 repressed cell proliferation during 24, 48, 72 h detected by MTS assay. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01. f BrdU incorporation assay of cells with knockdown of GOLPH3. Data shown are means ± SD (n = 5). **P < 0.01.g Flow cytometry assay showed the cell cycle arrest at G1 phase by knockdown GOLPH3. Data shown are means ± SD (n = 5). h Expression level of mRNA of cell cycle-related genes. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01 versus the corresponding control