Fig. 1

Investigation of CK1α RNA expression as a prognostic marker in CRC. a Box plot representing group comparison of relative CK1α RNA expression in normal and tumor tissue of CRC patients. b Kaplan-Meier plot displaying the overall survival of whole cohort, divided according to relative CK1α RNA expression in tumor tissue. *p < 0.05. CK1α RNA expression in colorectal tumor tissue of whole cohort was relatively quantified by qPCR using specific primers. HPRT gene served as reference gene. Graphs were created using IBM SPSS Statistics 20. c Analysis of disease-free survival (DFS) of CRC patients. Kaplan-Meier plot mapping DSF of whole cohort according to relative CK1α RNA expression in tumor tissue. *p < 0.05. CK1α RNA expression in colorectal tumor tissue of whole cohort was relatively quantified by qPCR using specific primers. HPRT gene served as reference gene. Graphs were created using IBM SPSS Statistics 20. d Western Blot Analysis of CK1α protein expression in colorectal normal and tumor tissue. CRC patients of the described cohort were randomly chosen and their normal and tumor tissue subjected to Western Blot analysis. Chemiluminescent blots were visualized on films and densitometry of bands analyzed using ImageJ software. Difference between relative density of tumor tissue and normal tissue was calculated and the entirety of values separated by the median in protein expression of tumor tissue lower/equal and higher/equal, respectively, to normal tissue. Data was drawn as a graph using GraphPad Prism 6 software