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Table 2 Immunohistochemical procedures

From: Analysis of tumour-infiltrating lymphocytes reveals two new biologically different subgroups of breast ductal carcinoma in situ

Ag Ab clone Ab supplier Ag retrieval Ab dilution incubation time Reveal system
ER SP1 Ventana CC2 36 min ready-to-use, 32 min ultraView DAB
PR 1E2 Ventana CC2 36 min ready-to-use, 20 min ultraView DAB
HER2 4B5 Ventana CC2 36 min ready-to-use, 20 min ultraView DAB
Ki67 SP6 Thermo Scientific CC2 36 min 1/100, 60 min ultraView DAB
CD8 SP16 Thermo Scientific CC2 36 min 1/400, 60 min ultraView DAB
CD4 SP35 Cell Marque CC2 36 min 1/50, 60 min ultraView DAB
FoxP3 2A11G9 Novus Biologicals CC2 36 min 1/25, 60 min ultraView Red
CD38 SP149 Cell Marque CC2 36 min 1/50, 60 min ultraView DAB
CD20 L26 Dako CC2 36 min 1/100, 32 min ultraView DAB
  1. Abbreviations: Ag antigen, Ab antibody, ER oestrogen receptor, PR progesterone receptor, CC1 or CC2 Cell Conditioning Buffer 1 or 2 (Ventana). All ultraView systems are from Ventana