Fig. 6

XPC knock down has small but significant impact on combined treatment. Melanoma cell lines Me4405 and Mel-RM were treated with combined decitabine (0.26 μM) and carboplatin (8 μg/mL) in the presence of XPC siRNA or non-targeting control (NTC). Knockdown of XPC was confirmed at the transcript by qPCR (a) and protein level by western blot (d). Apoptosis (b) was quantified to identify the effect of XPC knockdown on the response to combined treatment. Mean cell proliferation (c) for Me4405 and Mel-RM was quantified to further examine the effect of XPC knockdown on the response to combined treatment. Data represents mean of triplicate experiments, bars = SEM, *p < 0.05, **p < 0.01