Fig. 3

Knockdown of TSP50 inhibits proliferation, migration and invasion in BGC-823 cell. a The efficiency of shTSP50 transfection was confirmed by western blot. β-actin served as an inner control. b BrdU assay showed a significant decrease in BrdU uptake in shTSP50 cell compared to that of the control groups. (** P < 0.01). c Cell count experiment detected remarkable reduction of cell number in shTSP50 cell compared to that of the control groups. (** P < 0.01). d In wound closure assay, the migratory speed of shTSP50 cell decreased significantly compared to that of the control groups. (** P < 0.01). e In the transwell assay, migrated cells significantly reduced after transfected with shTSP50. (** P < 0.01). In the transwell invasion assay, invasiveness was quantified by cells through Matigel and it was showed fewer cells in shTSP50 BGC-823 cell than control groups. (** P < 0.01). All data are expressed as the mean ± SD from three independent experiments. Scale bar = 100 μm