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Fig. 2 | BMC Cancer

Fig. 2

From: Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer

Fig. 2

Overexpression of TSP50 promotes proliferation, migration and invasion in MGC-803 cell. a MGC-803 cell transfected with TSP50 overexpression plasmid. The efficiency of transfection was confirmed by western blot. β-actin served as an inner control. b BrdU assay showed a significant increase in BrdU uptake in MGC-803 cell transfected with TSP50 overexpression plasmid compared to that of the control group. (** P < 0.01). c Cell count experiment detected remarkable increase of cell number in overexpression-TSP50 MGC-803 cell compared to that of the control group. (** P < 0.01). d In wound closure assay, the migratory speed of MGC-803 cell transfected with TSP50 overexpression plasmid increased significantly compared to that of the vector control. (** P < 0.01). e In the transwell assay, migrated cells significantly increased after transfected with TSP50 overexpression plasmid. (* P < 0.05). In the transwell invasion assay, invasiveness was quantified by cells through Matigel and it was showed more cells in MGC-803 cell transfected with TSP50 overexpression plasmid than vector control. (* P < 0.05). All data are expressed as the mean ± SD from three independent experiments. Scale bar = 100 μm

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