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Fig. 4 | BMC Cancer

Fig. 4

From: Leptin-induced ER-α-positive breast cancer cell viability and migration is mediated by suppressing CCN5-signaling via activating JAK/AKT/STAT-pathway

Fig. 4

hrCCN5 protein reprograms leptin-induced epithelial to mesenchymal transition and migratory behavior. a Equal amount of protein lysates of MCF-7 cells treated with leptin in the presence or absence of hrCCN5 was loaded on 7.5–10% SDS-PAGE for the detection of EMT markers. A right panel shows the error bars which indicate mean ± SEM, and represents at least three independent experiments. b A diagram depicting experimental design to determine the leptin effect on BC cell migration in the presence or absence of hrCCN5 (left panel). MCF-7 and ZR-75-1 cells were treated with leptin in the presence or absence of hrCCN5 for 48 h and then seeded on the transwell filter insert of the modified Boyden chambers. Next day, the migrated cells were stained with crystal violet and quantitated on a microplate reader at 600 nm (right panel). The result is a representative of three independent experiments and displayed as mean ± SEM

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