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Fig. 3 | BMC Cancer

Fig. 3

From: Variant isoforms of CD44 involves acquisition of chemoresistance to cisplatin and has potential as a novel indicator for identifying a cisplatin-resistant population in urothelial cancer

Fig. 3

Protein expression of CD44v8–10 and xCT, and cytotoxic effects of CDDP in T24 and T24PR cells. a Western blot analysis of protein expression of CD44v8–10 and xCT, a subunit of the cystine transporter, in T24 cells and T24PR cells which was generated to acquire resistance to CDDP from T24 cells in our laboratory and these signal intensities. Left panel) Western blot analysis shows the stronger expression of CD44v8–10 and xCT proteins in T24PR cells as compared to those in T24 cells. Right panel) The signal intensities of the protein expression of CD44v8–10 and xCT in T24PR cells were significantly higher than those in T24 cells (p < 0.001 for both protein expression). b Immunofluorescence staining of CD44v8–10 expression in T24 and T24PR cells. Immunofluorescence staining shows CD44v8–10 protein expression in T24PR cells was stronger than that in T24 cells. c Cell viability relative to control at various concentrations of CDDP (0 to 10 μM) in (a) T24 cells and (b) T24PR cells. A cell viability assay showed that the IC50 of CDDP in T24PR cells was about 5-fold higher than that in T24 cells (T24: 4.1 μM, T24PR: 19.3 μM). †; p < 0.01, #; p < 0.001, compared with vehicle control (without CDDP exposure)

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