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Fig. 3 | BMC Cancer

Fig. 3

From: Monitoring the responsiveness of T and antigen presenting cell compartments in breast cancer patients is useful to predict clinical tumor response to neoadjuvant chemotherapy

Fig. 3

Suppressed T cell responsiveness in BC patients before chemotherapy. a Representative contour plots of T cells (SSC-A vs. CD3+) of HD unstimulated or stimulated 24 h with anti-CD3/CD28/CD2 beads (numbers represent MFI of CD3). b Quantification of CD3 MFI in PBMCs of HD in response to in vitro stimulation (left panel), and delta of CD3 MFI from HD (white box), and BC patients before (gray box) and after chemotherapy (dashed box – right panel). c Representative contour plots of T cell (gated on CD3+) activation phenotype (CD154 vs. CD69) of cells obtained from HD in response to in vitro stimulation with anti-CD3/CD28/CD2 beads, numbers represent the percentage of each population. d Quantification of MFI of each activation marker (delta of stimulated minus unstimulated cells) of CD25 (left panel), CD69 (middle panel) and CD154 (right panel) of HD (white box), and BC patients before (gray box) and after chemotherapy (dashed box). Box and whiskers 10–90%. HD (n = 12), patients before (n = 17) and after chemotherapy (n = 17). Non-parametric t-test (panel B – left panel) and Two-way ANOVA analysis, with Turkey’s multiple comparison tests, * p < 0.05, ** p < 0.01

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BMC Cancer

ISSN: 1471-2407

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