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Fig. 4 | BMC Cancer

Fig. 4

From: Optimising the chick chorioallantoic membrane xenograft model of neuroblastoma for drug delivery

Fig. 4

Tumour formation in chick embryo model. a Tumour formation in the chick embryo with and without the addition of trypsin/EDTA. Percentage tumour formation was calculated by dividing the number of eggs with tumours at E14 by the total number of embryos surviving until that time (between 30 and 40 embryos for each cell line and condition were analysed). b GFP images showing IMR-32 BE(2)C and Kelly tumours that have formed under the CAM. Note the smaller size of the Kelly tumours. c dissected IMR-32 and BE(2)C tumours viewed with GFP fluorescence with their corresponding bright field image. Note the chick CAM tissue that surrounds each tumour. d H&E staining of BE(2)C tumour FFPE sections. Black arrows indicate chick tissue. White arrows indicate tumour tissue. Scale bar is 250 μM

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