Fig. 5
From: Mechanism of recipient cell-dependent differences in exosome uptake
Different exosome uptake mechanisms among the three cell lines. Exosomes were isolated from COLO205 (a) and A549 cells (b) using the ultracentrifugation method. A549, HCT116 and COLO205 cells were treated with 200 μM genistein or 10 μM Pitstop 2 for 10 min and then incubated with DiO-labeled exosomes for 3 h. Uptake of DiO-labeled exosomes was analyzed by immunofluorescence microscopy. Representative images are shown. Immunofluorescence intensities of DiO-labeled exosomes per cell were analyzed using ImageJ software. Data are shown as the percentage of exosome uptake relative to the vehicle-treated cells and are expressed as means ± SEM (n = 5–10). *P < 0.05; **P < 0.01; NS: not significant