Fig. 2
From: Mechanism of recipient cell-dependent differences in exosome uptake
Uptake of DiO-labeled A549-, HCT116- and COLO205-derived exosomes into the individual donor cells. Exosomes were isolated from A549 (a), HCT116 (b) and COLO205 (c) cells using the ultracentrifugation method. Uptake of DiO-labeled exosomes at 37 °C (a-d) or 4 °C (d) was analyzed by immunofluorescence microscopy. Representative images are shown. Magnified images represent high-magnification images of the boxed area (a-c). Immunofluorescence intensities of DiO-labeled exosomes per cell were analyzed using ImageJ software (d). Data are expressed as means ± SEM (n = 5). *P < 0.05; **P < 0.01; NS: not significant