Fig. 7

Mitochondrial membrane potential and ROS production of HeLa cells treated with I-CRP. a Mitochondrial membrane potential was measured by fluorescence microscopy (25X) using TMRE staining in non-treated cells (control) or treated with I-CRP (1.25 U/mL). b Mitochondrial membrane potential was measured by flow cytometry through TMRE staining in cells left alone or treated with I-CRP (1.25 U/mL) for 24 h. c ROS levels were measured by fluorescence microscopy (25X) using DCFDA staining in non-treated cells (control) or treated with I-CRP (1.25 U/mL). d ROS levels were measured by flow cytometry through DCFDA staining in cells left alone or treated with I-CRP (1.25 U/mL) for 24 h