Fig. 6

Caspase-3 activity and effects of pan-caspase inhibition on HeLa cells treated with I-CRP. a Caspase-3 activation was measured by flow cytometry in cells that were left alone or pretreated with QVD.oph and then treated with I-CRP (1.25 U/ml) or etoposide (100 μM) for 24 h, data was then analyzed and graphed. b Cell viability was determined by flow cytometry in cells that were left alone or pretreated with QVD.oph and then treated with I-CRP (1.25 U/ml) or etoposide (100 μM) for 24 h. The percentages refer to Annexin-V-positive or Annexin-V-positive/PI-positive staining