Fig. 4

Cell cycle changes after I-CRP treatment of HeLa cells. a Schematic representation of changes in cellular DNA content in cells treated with different concentrations of I-CRP (1, 1.25, 2 U/mL) for 24 h, stained with propidium iodide and analyzed by flow cytometry to determine the cell-cycle distribution (left). The results were analyzed using Flowjo software and graphed (right). b Cell-cycle distribution of cells treated with I-CRP (1.25 U/mL) for different times (4, 8 16, 24, 48 h). c Cells were treated with I-CRP (1.25 U/mL) or etoposide (100 μM) for different times (4, 8 16, 24, 48 h) and analyzed as in (A) and graphed