Fig. 9

Increased tumor growth correlates with the expression of FAM3B and anti-apoptotic proteins. a Representative images of immunohistochemistry showing the expression of PCNA, FAM3B, Bax and Bcl-2 proteins in tumor xenografts tumors derived from DU145/FAM3B and DU145/control cells, stained in dark-brown. b Quantitative RT-PCR genes showing FAM3B overexpression in tumor samples (right) accompanied by increased mRNA Bcl-2 gene expression (left). Y-axis bars show mean ± SD fold increased gene expression compared to the mean of control tumors, normalized by expression of the HPRT housekeeping gene, in three independent experiments. Statistical differences among groups were determined by Student’s t-test (*p < 0.05, **p < 0.01). c Pearson’s correlation test shows a high and statistically significant correlation between tumor mass (% body weight) and FAM3B and Bcl-2 gene expression (fold increase in gene expression). For this comparison we included the tumor mass of all animals. When r > 0.8 and p < 0.05, we considered correlation to be highly positive