Fig. 5
Expression of Bcl-2 and Bcl-XL anti-apoptotic genes correlates with FAM3B overexpression. Gene expression analysis at mRNA level in DU145/FAM3B cells by quantitative RT-PCR for (a) Bcl-2, (b) Bcl-XL, and (c) Bax. Y-axis of bars shows mean ± SD fold increase in gene expression compared to control cells, in three independent experiments. HPRT housekeeping gene was included as a normalizer control. d Representative images from western blotting analysis with specific antibodies for FAM3B, Bax, Bcl-2, Bcl-XL, and β-actin, which was included as a loading control. Lanes were obtained from the same membrane and were contiguous, except in second row (Bcl-XL). e The relative intensity of bands was calculated by densitometry and was assumed as gene expression at protein level. Y-axis of bars shows mean ± SD fold increase of relative protein level, in three independent experiments. In all cases, statistical differences were determined by Student’s t-test (*p < 0.05; **p < 0.01)