Fig. 2

Exogenous rhFAM3B and CM-FAM3B enhance cell viability of DU145 cells. Analysis of cell viability was performed in DU145 cells treated during 48 h with several concentrations of (a) rhFAM3B (0.1–1000 pM); (b) rhFAM3B (100 pM) + TNF-α (added at a final concentration as indicated) + CHX (1 mM). c Western blot of total cell lysate and supernatant (culture medium +0.5% SFB) from 293 T cells transfected with pcDNA-FAM3B plasmid. The anti-FAM3B antibody recognized cytoplasmic and secreted FAM3B protein in culture medium (CM-FAM3B). Cell viability of DU145 cells was measured after (d) 48 h with several dilutions of CM-FAM3B and (e) 24, 48 and 72 h with a 1:10 dilution of CM-FAM3B. The results are expressed as means of three independent experiments and as relative ratios to the CM and recombinant protein-free controls (* p < 0.05; ** p < 0.01)