Fig. 3

PAK4 silencing inhibits GC cell proliferation via MEK/ERK pathway in vitro. a MGC-803 and SGC-7901 cells were transfected with transfection reagent only (Mock) or PAK4 siRNA (50 nM), at 48 h post-transfection, protein expression of PAK4 and β-actin (internal control) was detected using western blot. b MGC-803 and SGC-7901 cells were transfected as in (a), then cell proliferation levels were detected using MTT assay at 0, 24, 48 and 72 h post-transfection. c MGC-803 and SGC-7901 cells were transfected with negative control (NC), miR-199a/b-3p mimics (50 nM), transfection reagent only (Mock) or PAK4 siRNA (50 nM), at 48 h post-transfection, protein expression of p-MEK, p-ERK and β-actin (internal control) was detected using western blot

Data are shown as the mean ± SD of three independent experiments. **P < 0.01; ***P < 0.001.