Fig. 1

miR-199a/b-3p inhibits GC cell proliferation in vitro. a GC tissues and the matched adjacent normal gastric mucosal tissues were collected from 20 patients. Their miR-199a/b-3p expression was detected using real-time PCR. b MiR-199a/b-3p expression in a normal gastric mucosa cell line GES-1, GC cell lines MGC-803 and SGC-7901 was detected using real-time PCR. c MGC-803 and SGC-7901 cells were transfected with negative control (NC) or miR-199a/b-3p mimics at a final concentration of 10 or 50 nM, at 48 h post-transfection, miR-199a/b-3p expression was detected using real-time PCR. d MGC-803 and SGC-7901 cells were transfected as in (c), then cell proliferation levels were detected using MTT assay at 0, 24, 48 and 72 h post-transfection. a Data are shown as the mean ± SD (n = 20). b-d Data are shown as the mean ± SD of three independent experiments. n.s., not significant. *P < 0.05; **P < 0.01; ***P < 0.001