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Fig. 2 | BMC Cancer

Fig. 2

From: Coordinated expression and genetic polymorphisms in Grainyhead-like genes in human non-melanoma skin cancers

Fig. 2

miR-21–3p may directly target GRHL1 by interacting with its 3’UTR. a Schematic representation of the predicted target site of miR-21–3p in the 3’UTR of GRHL1. b Firefly/Renilla luciferases activity was analyzed in HEK293T cells 24 h after transfection with indicated plasmids and miR-21–3p or negative control miR, n = 3 biological replicates, *P < 0.05, **P < 0.01, data are shown as mean ± SD. C) Expression of GRHL genes and HDAC8 control gene measured by real-time PCR after treatment of HaCaT cells with miR-21–3p mimic (c = 60 nM; experiment was performed three times in duplicates). HDAC8 served as a positive control, because its expression is inversely regulated by miR-21–3p, therefore the level of HDAC8 mRNA is expected to decrease following cell treatment with miR-21–3p mimic [14]. TBP was used as a reference gene and Student t-test was used to determine statistical significance. Data are shown as mean ± SD

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