Fig. 4

Effects of various signal pathway inhibitors on the number of neurospheres derived from CD133⁺ cells and the survival rate of C6 glioma cells. a Primary neurospheres derived from CD133⁺ were dissociated and cultured. They were then treated with EGFR inhibitors (PD103035 and PD168393), PI3K inhibitor (LY294002), Akt inhibitor (Akt inhibitor VIII), mTOR inhibitors (rapamycin, Pl103), JNK inhibitor (SP600125), MEK inhibitor (PD98059), cSrc inhibitor (PP2), p38 MEK inhibitor (SB203580), JAK inhibitor (AG490), STAT3 inhibitor (WP1006), TGFβ inhibitor (SB431542), or β-catenin inhibitor (FH535) for 24 h and number of neurospheres were measured. b CD133⁺ cells were treated with various signal pathway inhibitors for 48 h and the survival rate was measured using the WST-1 assay