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Fig. 1 | BMC Cancer

Fig. 1

From: Synergistic inhibition of tumor growth by combination treatment with drugs against different subpopulations of glioblastoma cells

Fig. 1

Analysis, sorting, and characterization of CD133 positive cells via flow cytometry in C6 glioma cells. a Flow cytometry analysis of CD133 expression in C6 glioma cells. We detected 1.21% CD133 positive cells in C6 glioma cells. ***p < 0.001 vs. control. b C6 glioma cells were sorted for CD133 expression using magnetic bead cell sorting. CD133+ cells were collected and cultured under the same conditions as those for unsorted parental cells. Purity of the CD133+ populations was 35.26% at the second sorting and 72.88% after the third sorting. c Primary neurospheres derived from CD133+ or CD133 cells of C6 glioma cells were dissociated and cultured. CD133+ cells generated a greater number of secondary neurospheres than did CD133 cell. ***p < 0.001 vs. CD133. Scale bar: 100 μm

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