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Table 2 Toxicity test and mortality rates at 36 °C from 48 hpf to 144 hpf

From: Improving zebrafish embryo xenotransplantation conditions by increasing incubation temperature and establishing a proliferation index with ZFtool

Conc.a 5-FU ICb 5-FU ICb
Control - 0/24 0/24
24 h 34 °C 36 °C
 250 μM 0/20 0/4 0/20 0/4
 500 μM 0/20 0/4 0/20 0/4
 1000 μM 0/20 0/4 0/20 0/4
 1500 μM 0/20 0/4 1/20 0/4
 2000 μM 1/20 0/4 2/20 0/4
48 h 34 °C 36 °C
 250 μM 0/20 0/4 2/20 0/4
 500 μM 0/20 0/4 0/20 0/4
 1000 μM 0/20 0/4 5/20 0/4
 1500 μM 2/20 0/4 5/20 0/4
 2000 μM 2/20 0/4 11/20 0/4
72 h 34 °C 36 °C
 250 μM 0/20 0/4 1/20 0/4
 500 μM 0/20 0/4 0/20 0/4
 1000 μM 0/20 0/4 3/20 0/4
 1500 μM 1/20 0/4 3/20 0/4
 2000 μM 1/20 0/4 10/20 0/4
96 h 34 °C 36 °C
 250 μM 0/20 1/4 2/20 0/4
 500 μM 0/20 0/4 0/20 0/4
 1000 μM 0/20 1/4 5/20 0/4
 1500 μM 2/20 0/4 6/20 1/4
 2000 μM 3/20 0/4 14/20 0/4
  1. aConc concentration, bIC internal control. Negative control embryos were assayed in a separate 24 well plate. Additionally, four negative internal controls were placed in 4 of the 24 wells in each 5-FU treated plate being the other 20 wells 5-FU treatment