Improving zebrafish embryo xenotransplantation conditions by increasing incubation temperature and establishing a proliferation index with ZFtool

Table 2 Toxicity test and mortality rates at 36 °C from 48 hpf to 144 hpf

Conc.^a	5-FU	IC^b	5-FU	IC^b
Control -	0/24		0/24
24 h	34 °C		36 °C
250 μM	0/20	0/4	0/20	0/4
500 μM	0/20	0/4	0/20	0/4
1000 μM	0/20	0/4	0/20	0/4
1500 μM	0/20	0/4	1/20	0/4
2000 μM	1/20	0/4	2/20	0/4
48 h	34 °C		36 °C
250 μM	0/20	0/4	2/20	0/4
500 μM	0/20	0/4	0/20	0/4
1000 μM	0/20	0/4	5/20	0/4
1500 μM	2/20	0/4	5/20	0/4
2000 μM	2/20	0/4	11/20	0/4
72 h	34 °C		36 °C
250 μM	0/20	0/4	1/20	0/4
500 μM	0/20	0/4	0/20	0/4
1000 μM	0/20	0/4	3/20	0/4
1500 μM	1/20	0/4	3/20	0/4
2000 μM	1/20	0/4	10/20	0/4
96 h	34 °C		36 °C
250 μM	0/20	1/4	2/20	0/4
500 μM	0/20	0/4	0/20	0/4
1000 μM	0/20	1/4	5/20	0/4
1500 μM	2/20	0/4	6/20	1/4
2000 μM	3/20	0/4	14/20	0/4

^aConc concentration, ^bIC internal control. Negative control embryos were assayed in a separate 24 well plate. Additionally, four negative internal controls were placed in 4 of the 24 wells in each 5-FU treated plate being the other 20 wells 5-FU treatment

ISSN: 1471-2407