Fig. 3
Proliferation assay in zebrafish injected with different cell numbers at 34 °C and 36 °C. a, b Initial injected cells proliferation index at an incubation temperature of 34 °C (a: 100-200 cells, P.I. = 0.4603; b: 400-500 cells; P.I. = 0.7196). c, d Initial injected cells proliferation index at an incubation temperature of 36 °C (c: 100-200 cells, P.I. = 2.7558; d: 400-500 cells, P.I. = 1.9558). Images are representative of each of the conditions assayed. All images are a superposition of a fluorescence field image over a bright field image. In all panels the left image is a 48 hpf or 0 hpi zebrafish embryo, and the right image is the same zebrafish embryo with 120 hpf or 72 hpi. Scale bar = 100 μm. P.I. = proliferation index. e Comparison between the initial number of cells injected (Low: 100-200 or High: 400-500) and their proliferation at two different temperatures tested (nrep = 20-50, ntotal = 207, ***p < 0.01)