Acacia hydaspica ethyl acetate extract protects against cisplatin-induced DNA damage, oxidative stress and testicular injuries in adult male rats

Table 4 Effect of cisplatin (CP) and different treatments of AHE on testicular tissue antioxidant enzymes and GSH profile

Group	GSH (μM/g tissue)	GR (nM/min/mg protein)	GST (nM/min/mg protein)	γ-GT (nM/min/mg Protein)	GPx (nM/min/mg Protein)
Control	17.84 ± 0.77	158.4 ± 1.09	133.1 ± 0.95	326.5 ± 0.44	131.5 ± 1.72
CP	8.48 ± 0.415^***	110.7 ± 1.29^***	96.14 ± 0.84^***	106.9 ± 0.59^***	69.42 ± 1.62^***
AHE alone	17.87 ± 0.54⁺⁺⁺	159.6 ± 1.19⁺⁺⁺	133.4 ± 1.25⁺⁺⁺	329.4 ± 0.90⁺⁺⁺	132.2 ± 0.98⁺⁺⁺
CP + AHE	11.24 ± 0.65^***,+,###	121.5 ± 1.36^***,+++,###	111.7 ± 0.98^***,+++,###	156.4 ± 0.49^***,+++,###	83.91 ± 1.06^***,+++,###
AHE + CP	16.06 ± 0.30⁺⁺⁺	150.1 ± 1.14^**,+++	128.4 ± 0.92^*,+++	309.1 ± 0.89^***,+++	126.2 ± 0.91⁺⁺⁺
CP + Sily	16.15 ± 0.50⁺⁺⁺	149.2 ± 1.72^**,+++	128.2 ± 0.83^*,+++	312.5 ± 1.03^***,+++	126.3 ± 1.25⁺⁺⁺

n = 6/group; values are expressed as mean ± SEM; ^{*, **, ***} indicate significance from the control group at p < 0.05, p < 0.001 and p < 0.0001 probability level, respectively; ⁺⁺⁺ indicate significance from the CP group at p < 0.0001 probability level; ### indicates comparison of AHE + CP pre-treated group vs. CP + AHE post-treated group at p < 0.0001 probability level. Non-significant difference (p > 0.05) was recorded between control and AHE alone treated group in all parameters (One way ANOVA followed by Tukey’s multiple comparison tests)

ISSN: 1471-2407