Fig. 7

Time-dependent inhibition of invasiveness of IM3 glioma cells by VX-745. Boyden chamber invasion assays were performed on untreated parental C6 cultures and IM3 glioblastoma cultures that were treated for 6 h with DMSO or 20 μM VX-745 at two different times: 12 h or 24 h after serum shock. Plotted values correspond to the ratio of invasion cell counts in each culture adjusted relative to the averages of time-matched C6 cells, which were set at 100%. Data are represented as means (±SEM; n = 8). Relative cell invasion in DMSO-treated IM3 glioma cultures was significantly greater at both time points than that observed in parental C6 cells, and in IM3 glioma cultures treated with VX-745 at 24 h after serum shock (*, T-test p < 0.05)