Fig. 4

VX-745 shortens the period of clock gene rhythms in cultured Per2^Luc SCN cells and Bmal1-dLuc fibroblasts. The left panels depict individual recordings of ensemble bioluminescence (expressed as detrended baseline-subtracted counts per second) from representative cultures of serum-shocked Per2^Luc SCN cells (a) and Bmal1-dLuc fibroblasts (b) treated with DMSO (CON) or 20 μM VX-745. Right panels show bar graph comparisons of the circadian period (mean + SEM) of ensemble clock gene rhythms in control (CON) (n = 6) and VX-745-treated (10 μM or 20 μM; n = 6) SCN cells and fibroblasts. Asterisks indicate that the period of the SCN PER2::LUC and fibroblast Bmal1-dLuc rhythms in VX-745-treated cultures was significantly different (p < 0.05) from that in DMSO controls