Fig. 9

Up-regulation of VEGFR2 expression by isoprenaline is dependent on plexin-A1 expression. a-b MGC803 cells were transfected with shRNA/GFP, followed by G418 selection for 1 month; most cells (approximately 84%) were successfully transfected, comparing the optical microscopic image (a) and the fluorescence microscopic image (b). c shRNA transfected MGC803 cells were treated with 0, 5, and 10 μM ISO for 3 h, and qRT-PCR analysis of the mRNA levels of plexin-A1 and VEGFR2 in the shRNA knockdown group (Siplexin-A1) and control shRNA group (SiNC) (d-e) The corresponding protein levels of plexin-A1 and VEGFR2 were analyzed by Western blot and quantified. Data represent mean ± SD (n = 3 for each group, *P < 0.05, **P < 0.01)