Fig. 8

Isoprenaline promotes VEGFR2 and plexin-A1 expression via β2-AR in human gastric cancer cell lines. a-d MGC803 and HGC27 cells were starved overnight and incubated with 10 μM ICI 118,551 hydrochloride (ICI) (a selective antagonist of β2-AR) for 1 h to block β2-AR, in the presence of 0, 5, and 10 μM ISO for 6 h; (a, c) protein levels of plexin-A1, VEGFR2, and β2-AR were analyzed with Western blot; (b, d) the relative protein expression levels of plexin-A1, VEGFR2, and β2-AR were quantified based on the protein expression levels of β-actin. Data represent means ± SD (n = 3 for each group,*P < 0.05, **P < 0.01)