Fig. 5

Isoprenaline promotes plexin-A1 expression in human gastric cancer cell lines. a-c MGC-803 and HGC-27 cells were treated with 0, 5 or 10 μM ISO for 3 h after serum starvation. a qRT-PCR analysis of plexin-A1 expression in MGC803 and HGC27 cells. b-c The expression of plexin-A1 protein was analyzed by Western blot and quantified. d-f MGC803 cells were starved overnight and treated with 5 μM ISO for 0, 1, 2, 3 and 6 h; the relative mRNA expression levels of plexin-A1 were analyzed using qRT-PCR (d) and the protein expression of plexin-A1 was analyzed by Western blot and quantified (e-f).Data represent mean ± SD (n = 3 for each group,*P < 0.05, **P < 0.01)