Fig. 4

Isoprenaline promote tumor cells VEGF secretion and plexin-A1 is induced by VEGF165 in both HUVECs and gastric cancer cells. a-b MGC803 and HGC27 cells were treated with 5 μM ISO for 3 h after serum starvation; Elisa assay showed VEGF expression intracellular or extracellular of gastric cancer cells. c, e HUVECs and MGC803 cells were treated with 0, 5 or 10 μM of VEGF165 for 24 h after serum starvation; plexin-A1protein expression was analyzed by Western blot. The relative expression levels of plexin-A1 were shown in (d, f). Data represent mean ± SD (n = 3 for each group, *P < 0.05, **P < 0.01)