Fig. 2

Plexin-A1 is co-localized with VEGFR2 in both gastric tumor cells and vascular endothelial cells. a-b Representative images of fluorescent double staining of plexin-A1 (in red color) and VEGFR2 (in green color) in the vascular endothelial cells within normal gastric tissues (a, no positive double staining) and within the gastric tumor (b, arrow indicates cancer cells that show positive co-localization of plexin-A1 and VEGFR2; arrowheads indicates vascular endothelial cells that show positive co-localization of plexin-A1 and VEGFR2). The lower 3 panels are the magnified region of panel. b: left, co-localization; middle, green color only; right, red color only. Scale bar, 25 μm. c-d Representative images of fluorescent double staining of plexin-A1 (in red color) and VEGFR2 (in green color) in the normal gastric tissues (c, no positive double staining) and the gastric tumor (d, arrow indicates cancer cells that show positive co-localization of plexin-A1 and VEGFR2). The lower 3 panels are the magnified region of panel: left, co-localization; middle, green color only; right, red color only. Scale bar, 25 μm