Table 1 Oligonucleotide sequences used in real-time PCR and siRNA-mediated knockdown assay
Assay | Gene | Sequence (5′ → 3′) |
|---|---|---|
Real-time PCR | ||
VEGF | F | GGA GAT CCT TCG AGG AGC ACT T |
R | GGC GAT TTA GCA GCA GAT ATA AGA A | |
PDGF | F | GCC GGT CCA GGT GAG AAA GAT TG |
R | GGG GCC GGC GGA TTC TCA | |
HIF-1α | F | CGG CGA AGC AAA GAG TCT GAA GT |
R | TCG CCG TCA TAT GTT AGC ACC AT | |
NRF2 | F | CTCAGCATGATGGACTTGGA |
R | TCTATGTCTTGCCTCCAAAGG | |
β-Actin | F | CTC CAT CCT GGC CTC GCT GT |
R | GCT GTC ACC TTC ACC GTT CC | |
HIF-1α siRNA | (1) | CCC ATT CCT CAT CCG TCAA AT |
(2) | AGT CGA CAC AGC CTC GAT ATG | |
Control siRNA | UUC UCC GAA CGU GUC ACG UTT | |
NRF2 siRNA | (1) | GAAGGCACAATGGAATTCAAT |
(2) | GCCTTACTCTCCCAGTGAATA | |
Control siRNA | TTCTCCGAACGTGTCACGT | |