Fig. 5

Regulation of the expression of VEGF,PDGF and HIF-1α in MSCs by the NRF2 signaling pathway. MSCs were pre-stimulated in advance by inflammatory cytokines IFN-γ and TNF-α for 12 h, then transfected with NRF2-siRNA sequence or the relative mock sequences. Total RNA was extracted at 24 h after infection. a Real-time PCR analysis of the mRNA expression of NRF2 in MSCs that received no pretreatment and in MSCs treated with control siRNA or NRF2 siRNA under inflammatory conditions (all groups were pretreated with IFN-γ and TNF-α). b, c and d Real-time PCR analysis of the mRNA and Western blot analysis of protein expression of VEGF, PDGF and HIF-1α in MSCs that treated with control siRNA or NRF2 siRNA under conditions pretreated with or without IFN-γ and TNF-α. ^* p < 0.05