Skip to main content

Table 2 PCR mixes and cycling conditions

From: First-decade patient with colorectal cancer carrying both germline and somatic mutations in APC gene

PCR condition

PCR mix

Cycling conditions

Cycle number

PCR Master Mix

Forward primer (1 μM)

Reverse primer (1 μM)

DNA (50 ng/μl)

Final volume

Denaturation

Annealing

Extension

A

10 μL

4 μL

4 μL

2 μL

20 μL

96 °C

58 °C

74 °C

40

30 SEC

30 SEC

30 SEC

B

10 μL

4 μL

4 μL

2 μL

20 μL

96 °C

60 °C

68 °C

35

15 SEC

15 SEC

15 SEC