From: First-decade patient with colorectal cancer carrying both germline and somatic mutations in APC gene
PCR condition | PCR mix | Cycling conditions | Cycle number | ||||||
---|---|---|---|---|---|---|---|---|---|
PCR Master Mix | Forward primer (1 μM) | Reverse primer (1 μM) | DNA (50 ng/μl) | Final volume | Denaturation | Annealing | Extension | ||
A | 10 μL | 4 μL | 4 μL | 2 μL | 20 μL | 96 °C | 58 °C | 74 °C | 40 |
30 SEC | 30 SEC | 30 SEC | |||||||
B | 10 μL | 4 μL | 4 μL | 2 μL | 20 μL | 96 °C | 60 °C | 68 °C | 35 |
15 SEC | 15 SEC | 15 SEC |