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Table 1 PCR primers, internal sequencing primers used for sequencing reactions and annealing temperatures for the amplification of each amplicon of APC gene

From: First-decade patient with colorectal cancer carrying both germline and somatic mutations in APC gene

Amplicon

Primer name

Primer sequence

Size (bp)

PCR condition (see Table 2)

APC-E15.1

(with M13 tail)

APC-E15.1 forward

5′- TGTAAAACGACGGCCAGTTGTGACCTTAATTTTGTGATCTCTTGAT -3′

634

A

APC-E15.1 reverse

5′- CAGGAAACAGCTATGACCCCAAACTTCTATCTTTTTCAGAACGA -3′

APC-E15.2

(with M13 tail)

APC-E15.2 forward

5′- TGTAAAACGACGGCCAGTCCCAGCTCCTCTTCATCAAGA -3′

668

A

APC-E15.2 reverse

5′- CAGGAAACAGCTATGACCTGTTTGGGTCTTGCCCATCTT -3′

APC-E15.3

(with M13 tail)

APC-E15.3 forward

5′- TGTAAAACGACGGCCAGTCAGATGAGCAGTTGAACTCTGGAA -3′

756

A

APC-E15.3 reverse

5′- CAGGAAACAGCTATGACCCAGCTGATGACAAAGATGATAATGAAC -3′

APC-E15.4

(with M13 tail)

APC-E15.4 forward

5′- TGTAAAACGACGGCCAGTCCACTTGCAAAGTTTCTTCTATTAACC -3′

708

A

APC-E15.4 reverse

5′- CAGGAAACAGCTATGACCGAAGAACCTGGACCCTCTGAACT -3′

APC-E15.5

(with M13 tail)

APC-E15.5 forward

5′- TGTAAAACGACGGCCAGTAATAAAGCACCTACTGCTGAAAAGAGA -3′

751

A

APC-E15.5 reverse

5′- CAGGAAACAGCTATGACCTTTTTCCTCCTTGAGCCTCATC -3′

APC-E15.6

(with M13 tail)

APC-E15.6 forward

5′- TGTAAAACGACGGCCAGTTCGAATCCCCTCCAAATGAG -3′

560

A

APC-E15.6 reverse

5′- CAGGAAACAGCTATGACCAGGCGTGTAATGATGAGGTGAA -3′

APC-E15.7

(with M13 tail)

APC-E15.7 forward

5′- TGTAAAACGACGGCCAGTTGATAAGCTCCCAAATAATGAAGATAGAG -3′

659

A

APC-E15.7 reverse

5′- CAGGAAACAGCTATGACCTTATACATTCCTGCAACAGGTCATC -3′

APC-E15.8

(with M13 tail)

APC-E15.8 forward

5′- TGTAAAACGACGGCCAGTTGTTGAAGATACCCCAGTTTGTT -3′

650

A

APC-E15.8 reverse

5′- CAGGAAACAGCTATGACCTTGTCCTGCCTCGAGAGATT -3′

APC-E15.9

(with M13 tail)

APC-E15.9 forward

5′- TGTAAAACGACGGCCAGTCAGGGGAGAAAAGTACATTGGA -3′

692

B

APC-E15.9 reverse

5′- CAGGAAACAGCTATGACCTCCTTTGGAGGCAGACTCAC -3′

APC-E15.10

(with M13 tail)

APC-E15.10 forward

5′- TGTAAAACGACGGCCAGTCAGATGAGCCAACAGAACCTT -3′

632

A

APC-E15.10 reverse

5′- CAGGAAACAGCTATGACCTCACTGGATTCTGATGAAGCA -3′

APC-E15.11

(with M13 tail)

APC-E15.11 forward

5′- TGTAAAACGACGGCCAGTCGTGAGCACAGCAAACATTC -3′

663

B

APC-E15.11 reverse

5′- CAGGAAACAGCTATGACCTTTGCTTGAGCTGCTAGAACTG -3′

APC-E15.12

(with M13 tail)

APC-E15.12 forward

5′- TGTAAAACGACGGCCAGTCCCCTGACCAAAAAGGAACT -3′

623

B

APC-E15.12 reverse

5′- CAGGAAACAGCTATGACCGAAGTTGGGATGGGATGCTA -3′