Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: Melanoma cells replicate through chemotherapy by reducing levels of key homologous recombination protein RAD51 and increasing expression of translesion synthesis DNA polymerase ζ

Fig. 2

The reduction in RAD51 levels after cisplatin treatment in melanoma cells is not seen in other cell types. Graphs show the expression of RAD51, determined by western blotting, in five human melanoma cell lines (A375, C32, G361, HBL and WM115), two human ovarian cancer cell lines (PEO1 and PEO4) and the immortalised human fibroblast line, MRC5v1. Cells were untreated, or treated with 0.3, 1 or 3 μM cisplatin for 24, 48 or 72 h. RAD51 expression is plotted relative to β-actin and normalised to the level in untreated cells. Results are the means from two separate determinations. Representative western blots themselves are shown in Additional file 1: Figure S1

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us