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Fig. 1 | BMC Cancer

Fig. 1

From: Melanoma cells replicate through chemotherapy by reducing levels of key homologous recombination protein RAD51 and increasing expression of translesion synthesis DNA polymerase ζ

Fig. 1

Reduced RAD51 levels in melanoma cells after cisplatin treatment. a Levels of Nucleotide Excision Repair proteins increase after cisplatin treatment of A375 melanoma cells, while levels of RAD51 decrease dramatically. Total protein extracts from untreated A375 human melanoma cells and cells treated with 6 μM cisplatin (CDDP) for 48 h were western blotted for the NER proteins, ERCC1 (33 kDa), XPF (104 kDa) and XPA (40 kDa) and the homologous recombination protein, RAD51 (37 kDa). β-actin (42 kDa) served as the loading control. b Cisplatin concentration- and treatment time-dependent decrease in RAD51 levels in A375 melanoma cells. Total protein extracts from untreated A375 human melanoma cells and cells treated with CDDP concentrations ranging from 0.3 to 6 μM for 48 and 72 h were western blotted for RAD51. β-actin served as the loading control. The positions of molecular weight markers (in kDa) adjacent to proteins of interest are shown

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BMC Cancer

ISSN: 1471-2407

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