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Fig. 6 | BMC Cancer

Fig. 6

From: Effect of siRNA-silencing of SALL2 gene on growth, migration and invasion of human ovarian carcinoma A2780 cells

Fig. 6

SALL2 silencing for 48 h can promote migratory and invasive behaviour of A2780 cells by upregulating MMP2/9 expression and activating PI3K/Akt. (a) The protein expression levels of MMP2 and MMP9 in the A2780 cells after SALL2 silencing was detected by Western blot analysis. (b) Protein expression levels of p-Akt and Akt in the A2780 cells after SALL2 silencing were investigated through Western blot analysis. (c) Western blot analysis was used to determine the protein expression levels of Akt and p-Akt in the A2780 cells after transfection with Scrambled siRNA or SALL2 siRNA and subsequently treated with 50 μM DMSO or LY294002 for 30 min. (*P < 0.05; SALL2 siRNA + LY294002 vs. SALL2 siRNA + DMSO; n = 3). (d) Transwell assay was used to assess the migration of A2780 cells after transfection with Scrambled siRNA or SALL2 siRNA and subsequently treated with 50 μM DMSO or LY294002 for 30 min, respectively. (*P < 0.05; SALL2 siRNA+ LY294002 vs SALL2 siRNA+ DMSO; n = 3). Photomicrographs at 200× magnification (stained with crystal violet)

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