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Fig. 2 | BMC Cancer

Fig. 2

From: Effect of siRNA-silencing of SALL2 gene on growth, migration and invasion of human ovarian carcinoma A2780 cells

Fig. 2

SALL2 downregulation after 48 h of siRNA treatment. (a) Fluorescence photography and (b) FCM were used to determine the transfection of positive control siRNA into A2780 cells. (c) The efficiency of SALL2 silencing by siRNA in the A2780 cells. SALL2 expression was detected by qRT-PCR. (d) Western blot analysis indicated SALL2 silencing at the protein level. (*P < 0.05, **P < 0.01, n = 3). (e) Confocal microscopic analyses confirmed the intracellular SALL2 expression in the Blank control, Scramble, and SALL2 siRNA groups. The cells in each group were stained with purified mouse anti-human SALL2 (1:50) mAb and then with the secondary CY5-conjugated goat anti-mouse antibody (red). The cell nuclei was stained using Hoechst 33,258 (blue). Scale bars, 10 μm

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