Fig. 3
From: Overexpression of Cullin7 is associated with hepatocellular carcinoma progression and pathogenesis
Upregulation of Cullin7 enhances the proliferation, migration, and invasion capacities of HCC cells. a Ectopic expression of Cullin7 in HepG2 cells analyzed by western blotting. Ī²-actin was used as a loading control. b The transfection efficiency of Cullin7 was analyzed by measuring transcript levels using qRT-PCR analyses in HepG2 cells. c Cell proliferation after Cullin7 overexpression in cells was measured using MTT assays. d Colony formation assays show that upregulation of Cullin7 promotes cell growth, and the summary graphs are presented for the colony formation assay that is outlined. e HepG2 Cullin7 and control vector cells were subjected to a wound healing assay. f Overexpression of Cullin7 promoted cell invasion and migration as determined by Transwell migration and Matrigel invasion assays. Quantification of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown as a proportion of the vector controls. Error bars represent the meanāĀ±āSD of three independent experiments