Fig. 5

The role of NF-κB p65 in genistein induced reversal of EMT in HT-29 cells. Western blot analysis were carried out to demonstrated the of expression NF-κB p65 and phosphorylation NF-κB p65 in the cells treated by genistein (200 μmol/L) and TNF-α (10 ng/mL) respectively for 48 h. Density of the bands were quantified by a densitometry analysis. Genistein treatment leads to the decrease of both NF-κB p65 and p-NF-κB p65 expressions. Data are presented after normalization by β-actin. The data shown are representative of three independent experiments. (*p < 0.05, vs control; ^# p < 0.05, vs GEN group)