Fig. 3

Effect of genistein and TNF-α on EMT-related markers, E/N-cadherin in HT-29 cells. a the protein expressions of E-cadherin in the cells treated with genistein (200 μmol/L) and TNF-α (10 ng/mL) respectively for 48 h were examined by immunofluorescence staining, bar = 50 μm (×400). b The percentage of E-cadherin positive cells per field were calculated in 3 different fields and represented by graphs (**p < 0.01, vs control). c Western blot analysis of E/N-cadherin expression in the cells treated by genistein (200 μmol/L) and TNF-α (10 ng/mL) respectively for 48 h. Density of the bands were quantified by a densitometry analysis. Data are presented after normalization by β-actin. The data shown are representative of three independent experiments. (*p < 0.05, **p < 0.01 vs control)