Fig. 4

PKCα mediates transcriptional repression of p120-catenin and post-transcriptional repression of E-cadherin. a E-cadherin and p120-catenin protein expression upon PKCα knockdown was determined by Western blots. Blots are representative of three independent replicates. β-actin was used as the loading control. Densitometry analysis of E-cadherin and p120-catenin is shown. b E-cadherin (CDH1) and p120-catenin (CTNND1) expression upon PKCα knockdown was measured by qRT-PCR. Experiments were done in endocrine resistant breast cancer (MCF7/PKCα) and basal A TNBC cell lines (HCC1143, HCC1937). Graphs represent the SEM of at least three independent biological replicates. Significance was determined by student t-tests. c Membrane localization of p120-catenin and E-cadherin upon PKCα knockdown in HCC1143 was assessed by immunofluorescent staining according to Materials and Methods. Cells were treated with either negative siRNA (siC) or siRNA targeting PKCα (siP) and membrane localization of p120-catenin and E-cadherin was evaluated at 72 and 96 h after transfection. Scale bar 10uM. Quantification of p120-catenin and E-cadherin immunofluorescence intensity is shown. Significance was determined by one way ANOVA. *, P < 0.05, ** P < 0.01 ***, P < 0.001 ****, P < 0.0001